Reporter

Part:BBa_K1584026:Design

Designed by: All of us   Group: iGEM15_Elan_Vital_Korea   (2015-08-31)


Constitutive LuxR with AHL-inducible GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 908
    Illegal BsaI.rc site found at 1635


Design Notes

In order to properly produce the LuxR protein, we put a terminator behind LuxR.

We also had two XbaI sites at J61100, so when we tried to cut using EcoRI and XbaI, there was a chance that the constitutive promoter could be eliminated, which we had to consider.


Source

We used Part J61100, a ribosome binding site from Part J23100, Part C0062 to promote C0062, Part K83017 to put a Terminator behind LuxR, and Part J37032 to produce GFP.

References